![]() In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times. Klenow Fragment (3 5 exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5 3 exonuclease activity and has mutations (D355A, E357A) which abolish the 3 5 exonuclease activity (1). Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. ![]() Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water. Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water. QIAamp DNA Blood Mini, Midi and Maxi Kits.QIAGEN Protease is supplied in the following QIAGEN kits: QIAGEN Protease is completely free of DNase and RNase activities. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. Proteinase K is supplied in the following QIAGEN kits: This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Soluble calcium is not essential for enzymatic activity. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times.
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